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2010/1 Module Catalogue
Module Provider: Biosciences Short Name: GTXM004
Level: M Module Co-ordinator: PRICE SC Prof (Biosciences)
Number of credits: 15 Number of ECTS credits: 7.5
Module Availability
18-22 October ‘10
Assessment Pattern
Unseen 2.5 h written examination on the Friday of the module (50%)
Home-based assignment to be submitted within ten weeks (50%)
Module Overview
Module Aims
Learning Outcomes

Learning Outcomes:



The aims of this module are that the students will be able to understand:


·         Interaction of chemicals and radiations with genetic material


·         Exogenous and endogenous activities


·         Methods for detection of lesions


·         DNA repair activity, methods for the detection of activity


·         Structure/activity relationships


·         Conversion of a DNA lesion into gene and chromosome changes


·         Detection methods such as SSCP RSM FISH, CGH. Shuttle vectors


·         Mutation databases, mutation types and spectra


·         Relationships with cancer databases


·         Gene expression models



On the successful completion of the module the students should be able to assess cardiogenicity study reports in the context of overall toxicological evaluation. This will require:


·         A critical  understanding of the processes involved in mutation, DNA repair and carcinogenesis


·         An understanding of the basis of mutagenicity screening procedures and their limitations


·         Extrapolation of experimental data to man.


·         The ability to integrate knowledge of mutagenesis, into the process of risk assessment


·         Critical Evaluation of the data.


Module Content

Module content:



·         DNA modifications by ionising and non-ionising radiations


·         Chemically induced lesions, small adducts as illustrated by the alkylating agents.


·         Endogenous lesions, e.g. oxidative damage, the spontaneous lesion background.


·         Bulky adducts, e.g. polycyclic aromatic hydrocarbons such as benzo(a)pyrene, aflatoxins, synthetic molecules.


·         DNA reactive anti-cancer drugs, e.g. cis-platin, tamoxifen, non-DNA reactive anti-cancer drugs, e.g vinca alkaloids, taxol.


·         Lesion and adduct detection methods, 32P postlabelling, comet assay, immunological methods radiolabelled compounds, mass spectrometry.


·         Replication fidelity, mismatch repair, sensitivity to colorectal cancer. Base excision repair.


·         Nucleotide excision repair, post-replication and double strand break repair


·         Repair defective mutations. Human repair syndromes and their implications.


·         Detection methods for gene and chromosome changes such as SSCP, RSM, FISH, CGH.


·         The conversion of DNA lesions into gene and chromosome changes.




Methods of Teaching/Learning
Selected Texts/Journals

Pre-course Reading :


·         Friedberg E.C., Walker , G.C., Siede, W., et al (2006) DNA Repair and Mutagenesis, 2nd edn, pub: ASM Press (ISBN 1-55581-319-4) [core reading for all modules of the MTP]


·         A number of relevant papers will be selected from the Mutation Research journal.



Last Updated
February 2010