· DNA modifications by ionising and non-ionising radiations
· Chemically induced lesions, small adducts as illustrated by the alkylating agents.
· Endogenous lesions, e.g. oxidative damage, the spontaneous lesion background.
· Bulky adducts, e.g. polycyclic aromatic hydrocarbons such as benzo(a)pyrene, aflatoxins, synthetic molecules.
· DNA reactive anti-cancer drugs, e.g. cis-platin, tamoxifen, non-DNA reactive anti-cancer drugs, e.g vinca alkaloids, taxol.
· Lesion and adduct detection methods, 32P postlabelling, comet assay, immunological methods radiolabelled compounds, mass spectrometry.
· Replication fidelity, mismatch repair, sensitivity to colorectal cancer. Base excision repair.
· Nucleotide excision repair, post-replication and double strand break repair
· Repair defective mutations. Human repair syndromes and their implications.
· Detection methods for gene and chromosome changes such as SSCP, RSM, FISH, CGH.
· The conversion of DNA lesions into gene and chromosome changes.