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| Module Availability |
| Semester One |
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| Assessment Pattern |
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Assessment:
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Coursework - 30% practical write-up (submission deadline 3 weeks after last practical session)
Examination - 70%
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| Module Overview |
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This module addresses the essential concepts and basic principles of molecular biology techniques that are required for Bioscience graduates. It demonstrates the application of the basic theories of molecular biology learnt in level 1, by combining both the theory behind and practical laboratory experience of basic molecular biology techniques – encompassing a practical cloning project. The module builds up to more advanced techniques for studying gene expression and gene variation, their application and their impact on both the biosciences and the environment. This will give students an appreciation of applied molecular biology research.
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| Prerequisites/Co-requisites |
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| Module Aims |
To advance the student's understanding of molecular biology especially in relation to
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The concepts and basic principles of gene hybridisation and cloning
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Laboratory analysis of gene structure and expression
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The use of internet resources in molecular biology
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The manufacture of recombinant DNA products
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The impact of genetically engineered organisms on the environment
To introduce the student to the basic practical techniques used in gene cloning and molecular biology. |
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| Learning Outcomes |
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On successful completion of this module you should have gained knowledge about and practical experience of techniques in molecular biology and be able to apply that knowledge to:-
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Devise strategies for the cloning, manipulation and analysis of specific nucleic acid sequences
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Grasp the basic concepts and techniques for transcriptomic and proteomic analysis
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Appreciate the basic principles and applications of animal transgenesis
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Use the internet as a tool for gaining insight in genetic function and malfunction
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Be able to perform cloning of DNA into a vector, transform E. coli with recombinant plasmid DNA, select transformants, extract and purify DNA, polymerase chain reaction, gel electrophoresis, restriction endonuclease digestion and data analysis
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| Module Content |
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Lecture No:
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Lecturer
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Topic
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BASIC METHODS
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1
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KS
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Introduction, nucleic acid biochemistry
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2
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MvS
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Nucleic acid hybridisation
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3
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MvS
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Cloning and cloning vectors
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4
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MvS
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Purification and electrophoresis of nucleic acid
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5
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MvS
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PCR: Principles
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6
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MvS
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PCR: Technologies
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7
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MvS
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Real-time PCR
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PRACTICAL
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8—10
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KS, LM
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PCR
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11—13
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KS, LM
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Gel electrophoresis, ligation and transformation
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14
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KS, LM
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Colony identification and plasmid culture
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15—17
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KS, LM
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Plasmid prep, restriction digest, and gel electrophoresis
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18-20
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KS, LM
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Gel electrophoresis and data analysis
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ANALYSIS OF GENE VARIATION AND EXPRESSION
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18
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MvS
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DNA sequencing
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19
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MvS
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Analysis of genetic variation
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20
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EL
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Internet resources
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21
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EL
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Bioinformatics
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22
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MvS
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Gene expression
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23
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MvS
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RNA analysis
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24
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CPS
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Array analysis
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25
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MvS
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Proteomics
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26
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MvS, EL, CPS
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Tutorial
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ADVANCED METHODS FOR STUDYING GENE EXPRESSION
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27
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NJP
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DNA-protein interactions
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28
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NJP
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Protein-protein interactions
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29
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NJP
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Protein expression and engineering
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30
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NJP
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Protein expression and engineering
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31
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NJP
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Transgenic animals
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32
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NJP
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Transgenic animals
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33
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NJP
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RNA interference
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34
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NJP,
MvS, KS , LM
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Tutorial
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| Methods of Teaching/Learning |
| Lectures, practical, tutorial |
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| Selected Texts/Journals |
"From genes to genomes", Dale J, von Schantz M, (2007) Wiley
"Recombinant DNA", Watson J D (2007) Freeman
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| Last Updated |
| 4 May 2011 |
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