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2011/2 Provisional Module Catalogue - UNDER CONSTRUCTION & SUBJECT TO CHANGE
 Module Code: BMS2036 Module Title: MOLECULAR BIOLOGY AND GENETICS 2
Module Provider: Biosciences Short Name: BMS2036
Level: HE2 Module Co-ordinator: SWALES KE Dr (Biosciences)
Number of credits: 15 Number of ECTS credits: 7.5
 
Module Availability
Semester One
Assessment Pattern

Assessment:

 

 

 

Coursework  - 30%  practical write-up  (submission deadline 3 weeks after last practical session)

Examination  -  70%

Module Overview

This module addresses the essential concepts and basic principles of molecular biology techniques that are required for Bioscience graduates. It demonstrates the application of the basic theories of molecular biology learnt in level 1, by combining both the theory behind and practical laboratory experience of basic molecular biology techniques – encompassing a practical cloning project. The module builds up to more advanced techniques for studying gene expression and gene variation, their application and their impact on both the biosciences and the environment.   This will give students an appreciation of applied molecular biology research.

 

Prerequisites/Co-requisites
Module Aims

To advance the student's understanding of molecular biology especially in relation to

  • The concepts and basic principles of gene hybridisation and cloning
  • Laboratory analysis of gene structure and expression
  • The use of internet resources in molecular biology
  • The manufacture of recombinant DNA products
  • The impact of genetically engineered organisms on the environment

To introduce the student to the basic practical techniques used in gene cloning and molecular biology.

Learning Outcomes

On successful completion of this module you should have gained knowledge about and practical experience of techniques in molecular biology and be able to apply that knowledge to:-

  • Devise strategies for the cloning, manipulation and analysis of specific nucleic acid sequences
  • Grasp the basic concepts and techniques for transcriptomic and proteomic analysis
  • Appreciate the basic principles and applications of animal transgenesis
  • Use the internet as a tool for gaining insight in genetic function and malfunction
  • Be able to perform cloning of DNA into a vector, transform E. coli with recombinant plasmid DNA, select transformants, extract and purify DNA, polymerase chain reaction, gel electrophoresis, restriction endonuclease digestion and data analysis
Module Content

Lecture No:

Lecturer

Topic

 

 

 

 

BASIC METHODS

1

KS

Introduction, nucleic acid biochemistry

2

MvS

Nucleic acid hybridisation

3

MvS

Cloning and cloning vectors

4

MvS

Purification and electrophoresis of nucleic acid

5

MvS

PCR: Principles

6

MvS

PCR: Technologies

7

MvS

Real-time PCR

 

 

 

 

PRACTICAL

8—10

KS, LM

PCR

11—13

KS, LM

Gel electrophoresis, ligation and transformation

14

KS, LM

Colony identification and plasmid culture

15—17

KS, LM

Plasmid prep, restriction digest, and gel electrophoresis

18-20

KS, LM

Gel electrophoresis and data analysis

 

 

 

 

ANALYSIS OF GENE VARIATION AND EXPRESSION

18

MvS

DNA sequencing

19

MvS

Analysis of genetic variation

20

EL

Internet resources

21

EL

Bioinformatics

22

MvS

Gene expression

23

MvS

RNA analysis

24

CPS

Array analysis

25

MvS

Proteomics

26

MvS, EL, CPS

Tutorial

 

 

ADVANCED METHODS FOR STUDYING GENE EXPRESSION

27

NJP

DNA-protein interactions

28

NJP

Protein-protein interactions

29

NJP

Protein expression and engineering

30

NJP

Protein expression and engineering

31

NJP

Transgenic animals

32

NJP

Transgenic animals

33

NJP

RNA interference

34

NJP,

MvS, KS , LM

 

 

Tutorial

Methods of Teaching/Learning
Lectures, practical, tutorial
Selected Texts/Journals

"From genes to genomes", Dale J, von Schantz M, (2007)  Wiley

 "Recombinant DNA", Watson J D (2007) Freeman

 

 

Last Updated
4 May 2011