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| Module Availability |
Semester One |
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| Assessment Pattern |
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Practical write-ups 15% (deadline 1 week after practical: weeks 7, 8 & 9)
MCQ test 15%
Examination 70% (to be sub-divided into three sections, of which students have to answer at least one question per section)
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| Module Overview |
This module is divided into three broad topics, namely Enzymes (function and utilization, including two practical sessions), Metabolic Control (dealing with the control of metabolic pathways by the hormones insulin and glucagon, using diabetes as an example of a disease where these control processes are lacking), and Cell Signalling, discussing plasma membrane and nuclear receptors (their biological functions and mechanisms of action)
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| Prerequisites/Co-requisites |
Biochemistry at level 1 |
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| Module Aims |
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To provide a theoretical understanding of the nature, catalytic and kinetic properties of enzymes
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To carry out a practical study of an enzyme's kinetic properties
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To critically assess aspects of glucose and lipid metabolism
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To discuss metabolic diseases in conjunction with metabolic pathways
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Review receptors and the pathways of intracellular signalling
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Provide an understanding of cellular signalling mechanisms and their role in regulating gene transcription
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| Learning Outcomes |
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Define and describe the biochemical roles of enzymes
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Define and describe enzymes from a catalytic, kinetic and thermodynamic perspective
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Interpret experimental data, as it relates to enzyme kinetics
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Define and describe mechanisms of the control of enzyme activity
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Define and describe glucose homeostasis and control of glucose metabolism
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Define and describe glucose metabolism in disease, e.g. diabetes
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Define and describe lipid metabolism, including the lipoprotein pathway and cholesterol metabolism
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Define and describe plasma membrane and nuclear receptors and their role in controlling gene transcription
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Define and describe intracellular signalling in health and disease
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| Module Content |
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Lectures
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Lecturer
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Topic
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1-8
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AET
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Biochemical roles of enzymes;
Enzyme catalytic mechanisms
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9-16
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CA-R
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Experimental design and data analysis; Enzyme mechanisms;
Enzyme kinetics and inhibition
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17-18
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CA-R
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Practical 1: Data analysis (computer-based, Austin Pearce rooms)
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19-21
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AET
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Practical 2: Determine Km and Vmax in presence and absence of inhibitor
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22-29
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AET
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Diabetes;
Glucose metabolism in health and disease
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30-33
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tbc
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Lipid metabolism in Health and Disease
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34-41
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RJ
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Cell signalling
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42
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AET & RJ
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MCQ test: lectures 22-41
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43
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AET
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Feedback and revision tutorial
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44
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CA-R
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Feedback and revision tutorial
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45
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tbc
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Feedback and revision tutorial
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46
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RJ
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Feedback and revision tutorial
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| Methods of Teaching/Learning |
| Lectures, practicals, tutorial. |
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| Selected Texts/Journals |
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Lippincott's Illustrated Reviews. Biochemistry, 5th Edition. Champe, Harvey and Ferrier (2010)
Lehninger. Principles of Biochemistry, 4th Edition, Nelson, D.L. and Cox, M.M. W.H. Freeman (2005)
Lodish et al., Molecular Cell Biology, 6th Edition (2007)
Alberts et al., Molecular Biology of the Cell, 5th Edition (2008)
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Fundamentals of Enzymology, The Cell and Molecular Biology of Catalytic Proteins. Price and Stevens, 3rd Edition, Oxford University Press (1999)
Binding and Kinetics for Molecular Biologists, James A. Goodrich and Jennifer F. Kugel, Cold Spring Harbor Laboratory Press
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| Last Updated |
4 May 2011 |
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